# Series 3A-1: Multiple stressor experiments on T. pseudonana (CCMP1014): Macronutrient concentrations # PI: U. Passow, N. D'Souza (UCSB), E. Laws (LSU) # version date: 2019-06-17 # ========================= Phase CO2 Temperature Day Replicate SOL_PO4 OL_PO4 EL_PO4 SOL_SiO4 OL_SiO4 EL_SiO4 SOL_NO3_NO2 OL_NO3_NO2 EL_NO3_NO2 ------------------------- Acclimation 410 15 D0 NA 17.15 17.15 17.15 173.60 173.60 173.60 277.40 277.40 277.40 Acclimation 410 15 D2 NA 15.61 14.75 14.81 177.62 174.14 175.73 283.77 268.50 270.58 Acclimation 410 15 D4 NA 14.30 8.90 10.28 179.84 118.09 132.86 277.93 215.99 233.47 Acclimation 410 20 D0 NA 15.15 15.15 15.15 132.46 132.46 132.46 251.67 251.67 251.67 Acclimation 410 20 D2 NA 14.91 10.45 10.65 186.81 138.48 145.46 295.30 252.66 253.66 Acclimation 410 20 D4 NA 11.96 0.13 0.10 169.45 0.42 0.29 275.12 2.30 1.85 Acclimation 410 25 D0 NA 12.38 12.38 12.38 173.60 173.60 173.60 277.40 277.40 277.40 Acclimation 410 25 D2 NA 15.38 13.77 13.06 194.32 176.79 173.35 277.40 265.92 259.25 Acclimation 410 25 D4 NA 14.58 4.87 0.01 200.84 87.72 0.40 281.64 184.03 21.33 Acclimation 410 30 D0 NA 10.14 10.14 10.14 150.58 150.58 150.58 275.64 275.64 275.64 Acclimation 410 30 D2 NA 14.86 9.23 8.77 197.60 155.83 149.62 294.76 254.66 250.67 Acclimation 410 30 D4 NA 13.64 0.07 0.06 203.56 0.60 0.29 298.01 2.22 1.91 Acclimation 750 15 D0 NA 34.72 34.72 34.72 264.89 264.89 264.89 588.63 588.63 588.63 Acclimation 750 15 D2 NA 32.20 30.49 31.06 282.08 266.57 271.98 592.41 567.50 582.36 Acclimation 750 15 D4 NA 31.44 20.37 18.60 279.12 151.60 133.30 591.15 452.37 437.31 Acclimation 750 20 D0 NA 37.51 37.51 37.51 247.92 247.92 247.92 681.83 681.83 681.83 Acclimation 750 20 D2 NA 33.50 30.77 31.74 272.14 244.90 247.62 664.51 622.92 629.95 Acclimation 750 20 D4 NA 33.50 13.12 4.91 289.81 79.06 9.70 662.38 436.34 329.20 Acclimation 750 25 D0 NA 32.01 32.01 32.01 247.63 247.63 247.63 584.86 584.86 584.86 Acclimation 750 25 D2 NA 32.39 28.62 28.06 285.52 248.41 245.68 601.29 544.47 545.67 Acclimation 750 25 D4 NA 32.20 0.09 0.17 292.66 0.50 0.43 611.56 128.43 22.85 Acclimation 750 30 D0 NA 22.96 22.96 22.96 239.57 239.57 239.57 634.31 634.31 634.31 Acclimation 750 30 D2 NA 33.70 30.77 30.00 278.49 256.96 254.09 660.64 625.03 620.53 Acclimation 750 30 D4 NA 34.09 2.72 0.04 300.78 63.56 0.39 683.01 388.64 178.04 Acclimation 1000 15 D0 NA 5.41 5.41 5.41 184.92 184.92 184.92 88.81 88.81 88.81 Acclimation 1000 15 D2 NA 4.81 4.49 4.33 194.69 185.97 193.63 113.28 106.28 108.02 Acclimation 1000 15 D4 NA 3.50 0.34 0.43 185.78 137.29 136.38 87.53 50.14 52.12 Acclimation 1000 20 D0 NA 5.55 5.55 5.55 173.92 173.92 173.92 100.02 100.02 100.02 Acclimation 1000 20 D2 NA 5.29 4.31 4.57 173.82 162.84 172.04 125.90 109.02 114.61 Acclimation 1000 20 D4 NA 3.82 1.34 0.74 160.23 127.79 105.64 100.32 66.68 48.43 Acclimation 1000 25 D0 NA 5.41 5.41 5.41 184.79 184.79 184.79 92.39 92.39 92.39 Acclimation 1000 25 D2 NA 4.67 3.56 3.95 199.93 187.94 191.23 110.59 97.70 99.36 Acclimation 1000 25 D4 NA 2.94 0.03 0.08 149.98 44.43 60.41 79.17 0.90 6.38 Acclimation 1000 30 D0 NA 5.50 5.50 5.50 167.23 167.23 167.23 94.82 94.82 94.82 Acclimation 1000 30 D2 NA 4.74 4.49 4.20 186.48 175.18 178.49 122.29 114.61 115.56 Acclimation 1000 30 D4 NA 3.84 0.16 0.15 176.76 126.98 125.31 107.19 46.08 45.13 Experiment 410 15 D0 NA 17.37 17.37 17.37 188.65 188.65 188.65 296.74 296.74 296.74 Experiment 410 15 D1 NA 15.25 15.20 15.14 186.28 182.79 181.00 290.76 285.91 284.84 Experiment 410 15 D2 NA 14.77 12.38 11.78 184.26 155.67 149.76 286.45 257.72 251.67 Experiment 410 15 D3 NA 13.86 4.75 3.18 178.89 74.87 56.66 285.91 176.46 152.09 Experiment 410 20 D0 NA 26.19 26.19 26.19 263.63 263.63 263.63 708.82 708.82 708.82 Experiment 410 20 D1 NA 23.32 19.52 17.93 261.70 236.82 224.87 626.70 620.48 617.38 Experiment 410 20 D2 NA 22.81 10.14 5.10 261.03 153.56 117.43 631.38 521.15 482.34 Experiment 410 20 D3 NA 21.49 0.05 0.05 253.37 0.38 0.12 625.14 259.70 221.29 Experiment 410 25 D0 NA 11.78 11.78 11.78 188.65 188.65 188.65 296.74 296.74 296.74 Experiment 410 25 D1 NA 15.70 13.38 12.17 197.97 168.69 167.76 291.84 271.63 269.54 Experiment 410 25 D2 NA 15.51 3.93 1.87 198.45 68.49 33.93 292.39 165.83 85.59 Experiment 410 25 D3 NA 15.21 0.05 0.04 198.93 0.31 0.17 296.74 8.27 2.58 Experiment 410 30 D0 NA 26.19 26.19 26.19 259.34 259.34 259.34 666.29 666.29 666.29 Experiment 410 30 D1 NA 19.83 16.41 14.33 245.55 231.00 215.63 491.90 622.03 561.56 Experiment 410 30 D2 NA 18.68 3.47 0.36 239.50 132.37 99.98 464.87 512.70 424.57 Experiment 410 30 D3 NA 17.67 0.02 0.03 229.90 0.50 0.24 442.53 283.53 235.02 Experiment 750 15 D0 NA 40.31 40.31 40.31 360.55 360.55 360.55 670.29 670.29 670.29 Experiment 750 15 D1 NA 36.49 35.50 36.49 345.15 331.21 338.56 635.12 611.56 624.57 Experiment 750 15 D2 NA 36.29 32.01 31.25 344.99 294.31 286.05 635.12 577.38 569.96 Experiment 750 15 D3 NA 35.50 25.32 19.81 340.20 214.82 155.72 633.79 498.98 444.81 Experiment 750 20 D0 NA 33.90 33.90 33.90 213.50 213.50 213.50 599.24 599.24 599.24 Experiment 750 20 D1 NA 34.29 31.35 30.39 279.94 234.09 226.62 654.24 601.94 600.36 Experiment 750 20 D2 NA 33.70 22.96 16.51 290.99 175.21 100.46 647.67 495.23 442.95 Experiment 750 20 D3 NA 33.50 2.63 0.04 286.01 1.47 0.16 656.03 280.17 124.19 Experiment 750 25 D0 NA 40.31 40.31 40.31 360.07 360.07 360.07 677.21 677.21 677.21 Experiment 750 25 D1 NA 35.89 32.01 30.11 341.14 313.48 303.37 635.12 597.48 581.11 Experiment 750 25 D2 NA 35.30 21.32 7.53 345.60 199.10 83.91 641.77 486.55 357.93 Experiment 750 25 D3 NA 35.30 0.07 0.16 344.51 0.60 0.22 644.45 237.52 19.16 Experiment 750 30 D0 NA 34.09 34.09 34.09 216.47 216.47 216.47 619.67 619.67 619.67 Experiment 750 30 D1 NA 34.29 29.05 25.13 286.41 236.14 214.96 662.44 587.40 568.33 Experiment 750 30 D2 NA 34.29 11.84 0.82 297.40 111.61 15.51 655.80 429.47 319.89 Experiment 750 30 D3 NA 34.49 0.04 0.23 305.59 0.19 0.50 668.80 118.04 2.47 Experiment 1000 15 D0 NA 5.74 5.74 5.74 229.60 229.60 229.60 95.20 95.20 95.20 Experiment 1000 15 D1 NA 4.16 3.77 3.33 196.48 220.59 188.55 83.88 82.02 72.94 Experiment 1000 15 D2 NA 4.12 1.84 1.28 222.11 201.24 195.12 92.90 57.14 53.97 Experiment 1000 15 D3 NA 3.35 0.07 0.07 208.79 125.69 109.07 84.42 2.44 1.43 Experiment 1000 20 D0 NA 17.81 17.81 17.81 178.94 178.94 178.94 284.12 284.12 284.12 Experiment 1000 20 D1 NA 10.34 12.44 12.46 181.12 176.85 182.71 218.24 259.10 265.85 Experiment 1000 20 D2 NA 9.91 10.52 9.22 184.35 167.46 159.04 216.05 243.53 237.99 Experiment 1000 20 D3 NA 8.92 5.70 2.48 185.89 122.55 73.34 214.75 207.84 133.97 Experiment 1000 25 D0 NA 5.69 5.69 5.69 232.19 232.19 232.19 94.66 94.66 94.66 Experiment 1000 25 D1 NA 3.62 0.81 0.38 189.79 211.76 209.10 70.90 69.92 62.78 Experiment 1000 25 D2 NA 3.72 0.10 0.14 194.80 149.64 124.94 90.62 16.77 3.86 Experiment 1000 25 D3 NA 2.95 0.06 0.13 197.03 66.50 63.90 81.81 3.03 2.06 Experiment 1000 30 D0 NA 17.68 17.68 17.68 178.94 178.94 178.94 283.12 283.12 283.12 Experiment 1000 30 D1 NA 8.79 11.88 10.43 191.76 172.38 179.03 198.53 261.50 262.46 Experiment 1000 30 D2 NA 8.61 6.35 4.51 192.44 131.68 131.64 198.95 213.88 213.88 Experiment 1000 30 D3 NA 8.35 0.12 0.1074 193.97 3.53 1.70 198.95 41.95 41.22 Detection limit NA NA NA NA 0.10 0.10 0.10 1.00 1.00 1.00 0.20 0.20 0.20